Journal: PLoS ONE

Article Title: Advanced molecular surveillance approaches for characterization of blood borne hepatitis viruses

doi: 10.1371/journal.pone.0236046

Figure Lengend Snippet: Israeli HBV/HDV mNGS and xGen metrics.

Article Snippet: Specimens in the HBV genotype panel were purchased from Boca Biolistics (Pompano Beach, FL) or collected from volunteer blood donors in Spain and Cameroon (HBV/HDV co-infected) to demonstrate probe efficacy on diverse strains.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Advanced molecular surveillance approaches for characterization of blood borne hepatitis viruses

doi: 10.1371/journal.pone.0236046

Figure Lengend Snippet: (A) HBV/HDV xGen probes tile HBV genotypes A-I with 2X coverage and HDV genotypes 1–8 with 1X coverage. (B) Representative genome coverage plots of NGS data for HBV (left) and HDV (right) strains from Cameroon. For HBV, mNGS reads are shown in orange and xGen reads in blue. For HDV, mNGS reads are shown in green and xGen reads in purple. (C) Histograms of coverage (top) and reads/million (bottom) on co-infections or HBV-only infections. Country, genotype, and viral load are listed beneath each plot with the same color scheme as in Fig 2B.

Article Snippet: Specimens in the HBV genotype panel were purchased from Boca Biolistics (Pompano Beach, FL) or collected from volunteer blood donors in Spain and Cameroon (HBV/HDV co-infected) to demonstrate probe efficacy on diverse strains.

Techniques:

Journal: PLoS ONE

Article Title: Advanced molecular surveillance approaches for characterization of blood borne hepatitis viruses

doi: 10.1371/journal.pone.0236046

Figure Lengend Snippet: HBV and HDV diversity panel results.

Article Snippet: Specimens in the HBV genotype panel were purchased from Boca Biolistics (Pompano Beach, FL) or collected from volunteer blood donors in Spain and Cameroon (HBV/HDV co-infected) to demonstrate probe efficacy on diverse strains.

Techniques:

Journal: PLoS ONE

Article Title: Advanced molecular surveillance approaches for characterization of blood borne hepatitis viruses

doi: 10.1371/journal.pone.0236046

Figure Lengend Snippet: (A) Coverage plot of HBV specimen, 2001149. mNGS reads are shown in orange and xGen reads in dark blue (upper panel). Genotype D1 reads are in gold and genotype B2 reads are in brown (lower panel). (B) Coverage plot of HDV specimen, 2001234. (C) Neighbor-joining phylogenetic tree of near full-length HDV sequences. New Israeli sequence branches are in red and reference strains are in black labeled with accession numbers. Bootstrap values of nodes >70 are shown.

Article Snippet: Specimens in the HBV genotype panel were purchased from Boca Biolistics (Pompano Beach, FL) or collected from volunteer blood donors in Spain and Cameroon (HBV/HDV co-infected) to demonstrate probe efficacy on diverse strains.

Techniques: Sequencing, Labeling